Introduction: carboclip is a recombinant peptide-n-glycosidase f (pngase f), from elizabethkingia sp, overexpressed in e coli that contains a hexahistidine tag. Compare pngase f (glycerol free) from new england biolabs on biocomparecom. Materials remove-it® pngase f ( neb #p0706 ) chitin magnetic beads ( neb #e8036 ) magnetic separation rack ( neb #s1506. Protocol: use of pngase f and other glycosidases pngase f sources: new england biolabs, catalog no p0704 or p0705 sigma, catalog no p7367 promega, catalog no v4831. A protocol for use of n-glycosidase f (pngase f), which catalyzes the cleavage of n-linked oligosaccharides.
Pngase f is a recombinant glycosidase cloned from elizabethkingia miricola and overexpressed in e coli pngase f has a molecular weight of 36kda. Rapigest f ssurfactant: an enabling tool for in-solution enzymatic protein digestions ying qing yu and martin gilar waters corporation, milford, ma, us. Citations - n-glycanase (pngase f) for pngase f digestion the prozyme deglycosylation chemistry was used according to the manufacturer's protocol” product. Protocol: use of pngase f and other glycosidases protocol: protease and phosphatase inhibitors protocol: use of pngase f and other glycosidases. Life science researchers welcome to biokÉ, we are specialists in the following 3 fields cell biology, protein analysis and genomics.
Pngase f part number e-png01 peptide n glycosidase f, n-glycosidase, pngase f qa-bio™ pngase f cleaves asparagine-linked (n-linked) oligosaccharides from. Pngase f prime™ n protein deglycosylation using recombinant pngase f prime™ note: the following protocols are intended as a general guide for protein. Pngase f procotol reactions may be scaled-up linearly to accommodate larger amounts of glycoprotein and larger reaction volumes optimal incubation times.
Pngase f primetm is a recombinant protein deglycosylation using recombinant pngase f primetm note: the following protocols are intended as a general guide. The biokÉ webshop is under construction until january 2nd, 2018 we wish you a successful 2018.
Recombinant f meningosepticum pngase f have you used recombinant f meningosepticum pngase f protein, cf protocols faqs receive email. Recombinant pngase f enzyme for cleavage of intact n-glycans cleaves between innermost glcnac and asn recombinant gene from elizabethkingia meningosepticum.
Pngase f (from flavobacterium meningosepticum, molecular weight 355k) is commercially available from a number of sources enzymes purified from culture filtrate, new.
Pngase f date june 2012 filter by: nature protocols vol 7 pages p1299 - p1310 protocol exchange issn: 2043-0116 about npg contact npg. E-png05 pngase f specifications - protocol quality & purity qa-bio pngase f is tested for contaminating protease as follows: 10 µg of denatured bsa is incubated at. Peptide-n-glycosidase f, also known as pngase f, is a recombinant amidase which is supplied glycerol free for optimal performance in hplc intensive methods pngase f. Rapid pngase f is an improved reagent that allows the complete and rapid deglycosylation of antibod-ies and fusion proteins in minutes reaction protocols.Download Pngase f procotol